ABSTRACT
PURPOSE: Transient receptor potential vanilloid 1 (TRPV1) is a ligand-gated nonselective cation channel, which can be activated by capsaicin and other noxious stimuli. Recently, an association between bone pain and TRPV1 has been reported. However, the influence of osteoporosis on TRPV1 in the sensory system innervating the femur has not been reported. MATERIALS AND METHODS: TRPV1-immunoreactive (ir) in dorsal root ganglia (DRG) neurons labeled with neurotracer [Fluoro-Gold (FG)] innervating the femurs of Sprague Dawley rats were examined in control, sham, and ovariectomized (OVX) rats. We evaluated osteoporosis in the femurs and compared the proportion of TRPV1-ir DRG neurons innervating femur between the 3 groups of rats. RESULTS: OVX rats showed osteoporotic cancellous bone in the femur. FG labeled neurons were distributed from L1 to L6 DRG, but there was no significant difference in the proportion of labeled neurons between the 3 groups (p>0.05). The proportions of FG labeled TRPV1-ir DRG neurons were 1.7%, 1.7%, and 2.8% of DRG neurons innervating the femur, in control, sham-operated, and OVX rats, respectively. The proportion of TRPV1-ir neurons in DRG innervating the femur in OVX rats was significantly higher than that in control and sham-operated rats (p<0.05). CONCLUSION: Under physiological conditions, DRG neurons innervating femurs in rats contain TRPV1. Osteoporosis increases the numbers of TRPV1-ir neurons in DRG innervating osteoporotic femurs in rats. These findings suggest that TRPV1 may have a role in sensory perception of osteoporotic femurs.
Subject(s)
Animals , Female , Rats , Femur/innervation , Ganglia, Spinal/metabolism , Lumbar Vertebrae/innervation , Neurons , Osteoporosis/complications , Rats, Sprague-Dawley , Stilbamidines , TRPV Cation Channels/metabolismABSTRACT
Se realizó un trabajo de investigación clínica en población de edad pediátrica atendida en un Hospital de Ortopedia, la cual requirió de tratamiento quirúrgico en miembros inferiores y para el logro del mismo se administró anestesia regional del tipo de bloqueo caudal, el cual fue estudiado, en cuanto a dos posiciones: Sims modificada (decúbito lateral y una de las extremidades flexionadas) y la posición genupectoral. El resto del método las dosis y concentraciones fueron similares en ambos grupos. Los resultados mostraron que la posición de Sims es la que permitió mayor altura metamérica.